Recombinant Hepatitis C Virus (HCV) NS-4a+b antigen (a.a 1658-1863) Fluorescein conjugated, 19 kDa and Beta-galactosidase (114 kDa) fused at the N-terminus, was expressed in E. coli, and puried in vitro using conventional chromatography techniques.
19 kDa (1658-1863aa)
> 95% pure by SDS-PAGE (Bradford et al)
1 mg/ml (OD280nm)
8M urea; 20mM Tris-HCl, pH 8.0; 10mM Beta-mercaptoethanol
2-8°C short term, -20°C long term
The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.
Hepatitis C Virus is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of Hepatitis C Virus with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
HCV; HCV NS4; Hepatitis C Virus nonstructural antigen 4; Non structural protein 4A; Non structural protein 4B; NS4A; NS4B; Flaviviridae; Hepacivirus; Hepatitis C virus; p27; p8; Hepatitis C Virus NS4