Intended Use
For quantitative detection of HMGB1 in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. ELISA Microplate(Dismountable), 8×12, 2-8°C/-20°C
2. Lyophilized Standard, 2vial, 2-8°C/-20°C
3. Sample Dilution Buffer, 20ml, 2-8°C
4. Biotin-labeled Antibody(Concentrated), 120ul, 2-8°C(Avoid Direct Light)
5. Antibody Dilution Buffer, 10ml, 2-8°C
6. HRP-Streptavidin Conjugate(SABC), 120ul, 2-8°C(Avoid Direct Light)
7. SABC Dilution Buffer, 10ml, 2-8°C
8. TMB Substrate, 10ml, 2-8°C(Avoid Direct Light)
9. Stop Solution, 10ml, 2-8°C
10. Wash Buffer(25×), 30ml, 2-8°C
11. Plate Sealer, 5pieces
12. Product Description, 1copy
Performance Characteristics
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% within the expiration date under appropriate storage condition.
To minimize extra influence on performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is strongly suggested that the same operator performs the whole assay from the beginning to the end.
Standard Curve
Regarding calculation, (the relative O.D.450) = (the O.D.450 of each well) – (the O.D.450 of blank well). The standard curve can be plotted as the relative O.D.450 of each standard solution (Y) vs. the respective concentration of the standard solution (X). The target concentration of the samples can be interpolated from the standard curve. It is recommended to use some professional software to do this calculation.
COA
Publication 
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