Regulatory status: For research use only, not for use in diagnostic procedures.

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serum, plasma
Species Reactivity
Intended Use
The Pig HMG1/HMGB1 ELISA Kit is a sandwich ELISA for the quantitative measurement of pig HMG1/HMGB1 in serum and plasma.
Contents of Kit
1. Coated 96-well Strip Plate: 1
2. Standard (Lyophilized): 2 vials
3. Sample Diluent: 1 vial x 20 mL
4. Assay Diluent A: 1 vial x 12 mL
5. Assay Diluent B: 1 vial x 12 mL
6. Detection Reagent A: 1 vial x 120 μL
7. Detection Reagent B: 1 vial x 120 μL
8. Wash Buffer (30x): 1 vial x 20 mL
9. TMB Substrate: 1 vial x 9 mL
10. Stop Solution: 1 vial x 6 mL
11. Adhesive Plate Sealers: 4
12. Instruction Manual: 1
Upon receipt the kit should be stored at 4°C if intended for use within 24 hours. Otherwise the Assay Plate, Standard, Detection Reagent A, and Detection Reagent B should be stored at -20°C. Avoid repeated freeze-thaw cycles. Store all other kit components at 4°C. The Substrate should never be frozen. Once individual reagents are opened it is recommended that the kit be used within 1 month. Unused Strip Plate wells should be stored at -20°C in a sealed bag containing desiccant in order to minimize exposure to moisture. Do not use the kit beyond its expiration date.
Intra-Assay CV (<10%); Inter-Assay CV (<12%)
Detection Range
15.63 - 1000 pg/ml
28.3 pg/ml
Standard Curve


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