Rabbit Anti-Tetanus Toxoid IgG ELISA Kit

Name: Rabbit Anti-Tetanus Toxoid IgG ELISA Kit
SKU: DEIASL273
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum

Species Reactivity

Rabbit

Detection Method

Indirect-ELISA

Intended Use

This kit is intended to detect anti-Tetanus Toxoid IgG antibody in rabbit serum post vaccination.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Coated microwell strips	96 wells	2-8°C
2	Rabbit Anti-Tetanus Toxoid Positive Calibrator	10000 U/ml 0.4 mL	2-8°C
3	Sample Diluent	30 mL	2-8°C
4	Conjugate	12 mL	2-8°C
5	Wash Buffer (20× concentrated)	60 mL	2-8°C
6	Substrate Solution	12 mL	2-8°C
7	Stop Solution	5 mL	2-8°C

Storage

All reagents should be stored at 2-8°C , and should not be used beyond the expiration date on the label. Once opened, microtitration strips may be stored at 2-8°C until the expiration date on the label, provided that desiccated conditions are maintained. Unused strips should be returned to their original foil pouch along with the sachet of desiccant. Opened pouches should be securely resealed by folding over the open end and securing it with adhesive tape.
The working strength Wash Buffer should not be stored for longer than 3 weeks at 2-8°C. It is recommended that Wash buffer be freshly diluted before each assay. If the working strength buffer becomes visibly cloudy or develops precipitate during the 3 weeks, do not use it.
Indications of Deterioration
The Rabbit Anti-Tetanus Toxoid Assay may be considered to have deteriorated if:
1. The kit fails to meet the required criteria for a valid test (see Quality Control).
2. Reagents becoming visibly cloudy or develop precipitate. Note: Concentrated Wash buffer, when cold, normally develops crystalline precipitates, which re-dissolve on heating at 37°C.
3. The Substrate Solution turns dark green. This is likely to be caused by contamination of the Substrate Solution.

General Description

Tetanus also called lockjaw is caused by neurotoxin from deep wound infection with Clostridium tetani. Symptoms include muscle spasms of the jaw and elsewhere in the body. These symptoms are caused by a toxin released from the bacteria. Vaccines have been developed with Tetanus Toxoid (inactivated Tetanus toxin) to protect from infection. Antibodies should be detectable in animals post vaccination.

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