Pseudotyped VSV-SARS-CoV-2 S-ΔG-EGFP (COV-PSV11)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Product Overview
Pseudotyped VSV-SARS-CoV-2 S-ΔG-GFP encodes the antigenomic-sense (or positive-sense) RNA of a replicaton-restricted recombinant vesicular stomatitis virus (rVSV) in which the glycoprotein (G) gene has been replaced with SARS-CoV-2 spike protein. Because the infectivity of Pseudotyped VSV-SARS-CoV-2 S-ΔG-GFP is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. The pseudotype VSV particles encode GFP together with the VSV nucleocapsid (N), phosphoprotein (P), glycoprotein (G), and large polymerase subunit (L) in their pVSV-ΔG vector. When the VSV pseudovirus infect the target cells, GFP expression is proportional to the number of cells that were infected.
1 mL
Store at -80°C. Multiple freeze/thaw cycles not recommended.
When using the virus, transfer the virus from the -80 ° C refrigerator and melt it in an ice bath.
Frozen on dry ice
Biosafety Level: BSL-2
It is the responsibility of the principal investigator to seek Institutional Biosafety Safety Committee approval for recombinant DNA, transgenic animal or infectious agent use within their laboratory spaces and maintain an Institutional Biosafety Safety Committee approval during the time period these materials are used.


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