Pseudotyped rSARS-CoV-2 Spike (COV-PS03)

Product Overview
Pseudotyped rSARS-CoV-2 Spike is a replication-restricted, recombinant pseudotyped lentiviral particles containing SARS-CoV-2 spike protein. Pseudovirus display antigenically correct spike protein pseudotyped on replication-incompetent virus particles that contain a heterologous lentiviral (HIV) core. Because the infectivity of Pseudotyped rSARS-CoV-2 is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices.
Application Notes
We recommended to use 10-20 uL pseudotyped virus per 1E+04 293T cells for in vitro assay. Due to differences in cell status,the best infection conditions and MOI should be determined by the end user.The virus can be diluted with cell culture medium if needed.
1 mL
Store at -80°C. Multiple freeze/thaw cycles not recommended.
When using the virus, transfer the virus from the -80 ° C refrigerator and melt it in an ice bath.
Frozen on dry ice
Biosafety Level:    BSL-2
It is the responsibility of the principal investigator to seek Institutional Biosafety Safety Committee approval for recombinant DNA, transgenic animal or infectious agent use within their laboratory spaces and maintain an Institutional Biosafety Safety Committee approval during the time period these materials are used.


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Effects of ventilation on the indoor spread of COVID-19


Authors: Bhagat, Rajesh K.; Davies Wykes, M. S.; Dalziel, Stuart B.; Linden, P. F.

Although the relative importance of airborne transmission of the SARS-CoV-2 virus is controversial, increasing evidence suggests that understanding airflows is important for estimation of the risk of contracting COVID-19. The data available so far indicate that indoor transmission of the virus far outstrips outdoor transmission, possibly due to longer exposure times and the decreased turbulence levels (and therefore dispersion) found indoors. In this paper we discuss the role of building ventilation on the possible pathways of airborne particles and examine the fluid mechanics of the processes involved.

Liver transplantation in a patient after COVID-19-Rapid loss of antibodies and prolonged viral RNA shedding


Authors: Niess, Hanno; Borner, Nikolaus; Muenchhoff, Maximilian; Khatamzas, Elham; Stangl, Manfred; Graf, Alex; Girl, Philipp; Georgi, Enrico; Koliogiannis, Dionysios; Denk, Gerald; Irlbeck, Michael; Werner, Jens; Guba, Markus

To date, little is known about the duration and effectiveness of immunity as well as possible adverse late effects after an infection with SARS-CoV-2. Thus it is unclear, when and if liver transplantation can be safely offered to patients who suffered from COVID-19. Here, we report on a successful liver transplantation shortly after convalescence from COVID-19 with subsequent partial seroreversion as well as recurrence and prolonged shedding of viral RNA.

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