Pseudotyped VSV-SARS-CoV-2 S-ΔG-mCherry (COV-PSV10)

Pseudotyped VSV-SARS-CoV-2 S-ΔG-mCherry

Product Overview
Pseudotyped VSV-SARS-CoV-2 S-ΔG-mCherry is a replication-restricted, recombinant pseudotyped VSV particles containing SARS-CoV-2 spike protein. Because the infectivity of Pseudotyped VSV-SARS-CoV-2 S-ΔG-mCherry is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. The pseudotype VSV particles encode mCherry in their pVSV-ΔG vector. When the VSV pseudotypes infect the target cells, mCherry expression is proportional to the number of cells that were infected.
Application Notes
We recommended to use 10-20 uL pseudotyped virus per 1E+04 293T cells for in vitro assay. Due to differences in cell status,the best infection conditions and MOI should be determined by the end user.The virus can be diluted with cell culture medium if needed.
1 mL
Store at -80°C. Multiple freeze/thaw cycles not recommended.
When using the virus, transfer the virus from the -80 ° C refrigerator and melt it in an ice bath.
Frozen on dry ice
Biosafety Level:    BSL-2
It is the responsibility of the principal investigator to seek Institutional Biosafety Safety Committee approval for recombinant DNA, transgenic animal or infectious agent use within their laboratory spaces and maintain an Institutional Biosafety Safety Committee approval during the time period these materials are used.


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Authors: [Anonymous]

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Value of swab types and collection time on SARS-COV-2 detection using RT-PCR assay


Authors: Liu, Min; Li, Qianyuan; Zhou, Jun; Ai, Wen; Zheng, Xiaoling; Zeng, Jingjing; Liu, Yuwen; Xiang, Xiying; Guo, Rong; Li, Xiaoyin; Wu, Xiandi; Xu, Haiying; Jiang, Ling; Zhang, Huaqin; Chen, Jing; Tian, Lili; Luo, Jun; Luo, Chunhua

Objective: Low viral load from patients infected with SARS-CoV-2 during infection late stage easily lead to false negative nucleic acid testing results, thus having great challenges to the prevention and control of the current pandemic. In present study, we mainly aimed to evaluate specimen types and specimen collection timepoint on the positive detection of 2019 novel coronavirus from patients at infection late stage based on RT-PCR testing. Methods: Paired nasopharyngeal swabs, nasal swabs, oropharyngeal swabs and anal swabs were collected from patients infected with SARS-CoV-2 during infection late stage before washing in the morning and afternoon on the same day. Then virus RNA was extracted and tested for 2019-nCoV identification by RT-PCR within 24 h. Results: Viral load was low at late infection stage. Specimens collected before washing in the morning would increase the detection ratio of 2019-nCoV. Detection ratio of nasopharyngeal swab [65 (95 % CI: 49.51-77.87) vs 42.5(95 % CI: 28.51-57.8)] or nasal swab [57.5 (95 % CI: 42.2-71.49) vs 35 (95 % CI: 22.13-50.49)] is higher not only than oropharyngeal swab[22.5 (95 % CI: 12.32-37.5) vs 7.5 (95 % CI: 2.58-19.86)], but also anal swab [2.5 (95 % CI: 0.44-12.88) vs 5 (95 % CI: 1.38-16.5)]. Conclusions: In summary, our research discovers that nasopharyngeal or nasal swab collected before washing in the morning might be more suitable for detecting of large-scale specimens from patients infected with low SARSCoV-2 load during infection late stage. Those results could facilitate other laboratories in collecting appropriate specimens for improving detection of SARS-CoV-2 from patients during infection late stage as well as initially screening.

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