Porcine OPG (Osteoprotegerin) ELISA Kit

Sample

serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Porcine

Detection Method

sELISA

Intended Use

For Research Use Only. Not For Use In Diagnostic Procedures. In vitro quantitative determination of OPG concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

The sealed kit can be stored at 2-8 °C. The storage condition for opened kit is specified in the table below:
1. ELISA Microplate(Dismountable), 8×6 (8×12 for 96T). Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
2. Lyophilized Standard, 1 vial (2 vial for 96T). Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
3. Biotin-labeled Antibody(Concentrated, 100X), 60ul (120ul for 96T). 2-8°C
4. HRP-Streptavidin Conjugate(SABC, 100X), 60ul (120ul for 96T). 2-8°C (Avoid Direct Light)
5. TMB Substrate, 5ml (10ml for 96T). 2-8°C
6. Sample Dilution Buffer, 10ml (20ml for 96T). 2-8°C
7. Antibody Dilution Buffer, 5ml (10ml for 96T). 2-8°C
8. SABC Dilution Buffer, 5ml (10ml for 96T). 2-8°C
9. Stop Solution, 5ml (10ml for 96T). 2-8°C
10. Wash Buffer(25X), 15ml (30ml for 96T). 2-8°C
11. Plate Sealer, 3 pieces (5 pieces for 96T)
12. Product Description, 1 copy

Storage

2-8°C(for sealed box), please do not freeze! See kit label for expiry date

Precision

Intra-assay Precision:
Sample 1 Mean=60.92 pg/ml, CV=5.02%;
Sample 2 Mean=258.9 pg/ml, CV=5.05%;
Sample 3 Mean=982.7 pg/ml, CV=5.27%.
Inter-assay Precision:
Sample 1 Mean=64.23 pg/ml, CV=4.96%;
Sample 2 Mean=251.8 pg/ml, CV=5.18%;
Sample 3 Mean=988.2 pg/ml, CV=5.25%.

Detection Range

31.25-2000 pg/ml

Detection Limit

18.75 pg/ml

Sensitivity

18.75 pg/ml

Standard Curve

1. Calculate the mean OD450 value (using the original OD450 value or the corrected OD450 value) of the duplicate readings for each standard, control, and sample. Then, obtain the value of calculation by subtracting the OD450 blank.
2. Create a four parameter logistic curve by plotting the mean absorbance for each standard on the y-axis against the concentration on the x-axis. (Remove the OD450 blank during plotting.) Alternatively, you can use the curve fitting software offered by the microplate reader.
3. Calculate the sample concentration by substituting OD450 value into the standard curve. Diluted samples should be multiplied by the relevant dilution ratio.