Human IgE ELISA Kit

Name: Human IgE ELISA Kit
SKU: DEIA9467
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum

Species Reactivity

Human

Detection Method

Sandwich-ELISA

Intended Use

The human IgE ELISA kit is for research use only. Under no circumstances may it be used for diagnostic purposes.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Anti Human IgE Coated 96-well Plate	12×8 wells	2-8°C
2	HRP Conjugate	11 ml	2-8°C
3	IgE Stock (lyophilized)	1	-20°C
4	20× Wash Solution	50 ml	2-8°C
5	10× Diluent	25 ml	2-8°C
6	TMB Reagent	11 ml	2-8°C
7	Stop Solution	11 ml	2-8°C

Storage

The lyophilized IgE stock should be stored in a freezer at -20°C when the kit is received. The rest of the kit should be stored in a refrigerator at 4°C. The kit will remain stable for at least six months from the date of purchase provided that the components are stored as described. The microtiter plate should be kept in a sealed bag with desiccant to minimize exposure to damp air.

Performance Characteristics

Parallelism: To assess performance of the assay, three human ascites samples with IgE concentrations of 10.28, 4.8 and 2.13 μg/ml were serially diluted to produce values within the dynamic range of the assay.

General Description

The human IgE ELISA kit is for measurement of IgE in human serum and fluids. The assay uses two monoclonal antibodies developed at CD; one for solid phase immobilization (microtiter wells), the other, conjugated to HRP, is used for detection.
IgE is the least abundant immunoglobulin in serum, typically present at levels of approximately 100 ng/ml. It is involved in allergic reactions, binding to Fc receptors on basophils and mast cells. Subsequent binding of antigen to IgE triggers release of histamine and other vasoactive amines. IgE levels are elevated in asthma, eczema, rhinitis, and parasitic infections.

Standard Curve

A typical standard curve with absorbance at 450nm on the Y-axis and IgE concentrations on the X-axis is shown below. This curve is
for the purpose of illustration only and should not be used to calculate unknowns. Each user should obtain data and standard curve in each experiment. Please note that we perform validation of all assays using shaking incubators at 150 rpm and 25°C. Performance of the assay at lower mixing speeds and temperatures may result in lower absorbance values.