Monkey IgE ELISA Kit

Sample

Serum

Species Reactivity

Monkey

Detection Method

Sandwich-ELISA

Intended Use

The monkey IgE ELISA kit is designed for measurement of IgE in rhesus and cynomolgus monkey serum.

Contents of Kit

Storage

The lyophilized IgE stock should be stored in a freezer at -20°C when the kit is received. The remainder of the kit should be stored in a refrigerator at 4°C and must not be frozen. The kit will remain stable for at least six months from the date of purchase provided that the components are stored as described. The microtiter plate 1 The IgE stock used in this kit is of non-monkey origin. It behaves identically to oldworld monkey IgE. The use of non-monkey IgE allows export of the kit without requirement for CITES documentation. should be kept in a sealed bag with desiccant to minimize exposure to damp air.

Performance Characteristics

Parallelism: To assess performance of the assay, two samples containing IgE at concentrations of 1010 and 477 ng/ml were serially diluted to produce values within the dynamic range of the assay.

General Description

IgE is the least abundant immunoglobulin in serum, typically present at levels of approximately 100 ng/ml. It is involved in allergic reactions, binding to Fc receptors on basophils and mast cells. Subsequent binding of antigen to IgE triggers release of histamine and other vasoactive amines. IgE levels are elevated in asthma, eczema, rhinitis, and parasitic infections.

Standard Curve

A typical standard curve with absorbance at 450nm on the Y-axis and IgE concentrations on the X-axis is shown below. This curve is for the purpose of illustration only and should not be used to calculate unknowns. Each user should obtain data and standard curve in each experiment. Please note that we perform validation of all assays using shaking incubators at 150 rpm and 25°C. Performance of the assay at lower mixing speeds and temperatures may result in lower absorbance values.

General Notes

IgE is the least abundant immunoglobulin in serum, typically present at levels of approximately 100 ng/ml. It is involved in allergic reactions, binding to Fc receptors on basophils and mast cells. Subsequent binding of antigen to IgE triggers release of histamine and other vasoactive amines. IgE levels are elevated in asthma, eczema, rhinitis, and parasitic infections. The monkey IgE ELISA kit is designed for measurement of IgE in rhesus and cynomolgus monkey serum.