Total human IgE ELISA Kit (DEIABL50)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
Plasma, Serum
Species Reactivity
Human
Intended Use
The total Human IgE test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgE in human biological samples.
Contents of Kit
1. DILUENT CONCENTRATE (Running Buffer)
2. WASH SOLUTION CONCENTRATE
3. ENZYME-ANTIBODY CONJUGATE 100X
4. CHROMOGEN-SUBSTRATE SOLUTION
5. STOP SOLUTION
6. ANTI-HUMAN IgE ELISA MICRO PLATE
7. HUMAN IgE CALIBRATOR
Storage
2-8°C
Detection Range
3.125 ng/ml - 200 ng/ml
Sensitivity
0.912 ng/ml
Reconstitution And Storage
The expiration date for the package is stated on the box label.
1. DILUENT
The 5X Diluent Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8°C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16-25°C) or at 4-8°C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-IgE conjugate should be stored at 4-8°C and diluted immediately prior to use. The working conjugate solution is stable for up to 1 hour when stored in the dark.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8°C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8°C and is stable until the expiration date.
6. ANTI-HUMAN IgE ELISA MICRO PLATE
Anti-Human IgE coated wells are stable until the expiration date, and should be stored at 4-8°C in sealed foil pouch with desiccant pack.
7. HUMAN IgE CALIBRATOR
Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4°C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
INDICATIONS OF INSTABILITY
If the test is performing correctly, the results observed with the standard solutions should be within 20 % of the expected values.

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