Chicken OPG (Osteoprotegerin) ELISA Kit

Sample

serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Chicken

Detection Method

sELISA

Intended Use

For Research Use Only. Not For Use In Diagnostic Procedures. In vitro quantitative determination of OPG concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

The sealed kit can be stored at 2-8 °C. The storage condition for opened kit is specified in the table below:
1. ELISA Microplate(Dismountable), 8×12 (8×6 for 48T). Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
2. Lyophilized Standard, 2 vial (1 vial for 48T). Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
3. Biotin-labeled Antibody(Concentrated, 100X), 120ul (60ul for 48T). 2-8°C
4. HRP-Streptavidin Conjugate(SABC, 100X), 120ul (60ul for 48T). 2-8°C (Avoid Direct Light)
5. TMB Substrate, 10ml (5ml for 48T). 2-8°C
6. Sample Dilution Buffer, 20ml (10ml for 48T). 2-8°C
7. Antibody Dilution Buffer, 10ml (5ml for 48T). 2-8°C
8. SABC Dilution Buffer, 10ml (5ml for 48T). 2-8°C
9. Stop Solution, 10ml (5ml for 48T). 2-8°C
10. Wash Buffer(25X), 30ml (15ml for 48T). 2-8°C
11. Plate Sealer, 5 pieces (3 pieces for 48T)
12. Product Description, 1 copy

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date

Precision

Intra-assay Precision:
Sample 1: Mean 58.65 pg/ml, SD 3.01, CV 5.13%
Sample 2: Mean 236.2 pg/ml, SD 12.19, CV 5.16%
Sample 3: Mean 965.5 pg/ml, SD 51.94, CV 5.38%

Inter-assay Precision:
Sample 1: Mean 59.13 pg/ml, SD 3.0, CV 5.07%
Sample 2: Mean 244.9 pg/ml, SD 12.96, CV 5.29%
Sample 3: Mean 980.1 pg/ml, SD 52.53, CV 5.36%

Detection Range

31.25-2000pg/ml

Sensitivity

18.75pg/ml

Standard Curve

1. Calculate the mean OD450 value (using the original OD450 value or the corrected OD450 value) of the duplicate readings for each standard, control, and sample. Then, obtain the value of calculation by subtracting the OD450 blank.
2. Create a four parameter logistic curve by plotting the mean absorbance for each standard on the y-axis against the concentration on the x-axis. (Remove the OD450 blank during plotting.) Alternatively, you can use the curve fitting software offered by the microplate reader
3. Calculate the sample concentration by substituting OD450 value into the standard curve. Diluted samples should be multiplied by the relevant dilution ratio.