General Notes
This product (Catalog No. DPATB-H83287) is a rabbit-derived IgG polyclonal antibody raised against c. tetani Tetanus toxin, targeting the amino acid region 801–900 of the protein. It has been affinity purified by Protein A. Tetanus toxin is also called spasmogenic toxin, tetanospasmin or abbreviated to TeTx or TeNT. The tetanus toxin protein has a molecular weight of 150kDa. It is translated from the TetX gene as one protein which is subsequently cleaved into two parts: a 100kDa H-chain or heavy chain or B-chain and a 50kDa amino terminal light or A-chain (C-fragment). The light chain contains the enzymatic portion of the toxin and is responsible for its toxic effects. The light chain activity is abolished by a mutation at His233 to either Cys or Val or Glu234 to Gln. H-chain further cleavage into Hn (amino terminal) and Hc (carboxy terminal) fragments. The chains are connected by a disulfide bond. The B-chain binds to dissialogangliosides (GD2 and GD1b) on the neuronal membrane and contains a translocation domain which aids the movement of the protein across that membrane and into the neuron. The A-chain, a zinc endopeptidase, attacks the vesicle-associated membrane protein (VAMP).
DPATB-H83287 is suitable for use in IHC-P (1:100–1:500), IHC-F (1:100–1:500), IF (immunofluorescence) (1:100–1:500), ICC/IF (1:100–1:500), ELISA (1:5,000–1:10,000) applications.
DPATB-H83287 is supplied as a liquid formulation in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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