Mouse Anti-Tetanus Toxin Monoclonal Antibody, clone IZC 279-22

Q: Our research group is planning to perform a competitive or sandwich Enzyme-Linked Immunosorbent Assay (ELISA) for tetanus toxin detection. We intend to use this product to sensitize the plate (capture antibody) and subsequently use a different monoclonal antibody as the primary detection antibody. To ensure the success of our immunoassay specifically to prevent competition between the two monoclonal antibodies it is crucial that they bind to distinct epitopes on the C. tetani Tetanus Toxin target.

A: The epitope specificity of CABT-NS1527 differs from that of CABT-NS1528 and CABT-NS1529, as determined by competitive ELISA. CABT-NS1527 and CABT-NS1530 has overlapping epitope binding sites as determined by competitive ELISA, where CABT-NS1530 inhibits the binding of CABT-NS1527.
CABT-NS1652 reacts well in ELISA with coated tetanus toxoid and in sandwich ELISA as catching antibody with monoclonal anti TeNT antibody CABT-NS1530 as detection antibody.