GST-human SREBP-2 fusion protein (amino acids 48 to 403)
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0-Ag14
Clone
IgG-2E3
Cell Line Description
Animals were immunized against the GST-human SREBP-2 fusion protein (amino acids 48 to 403). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody recognizes both the precursor and mature forms of SREBP-2 in cultured human cells
Application Notes
It can be used in WB and ELISA to study SREBP-2 in cultured human cells grown under different conditions.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Target
Synonyms
SREBF2; sterol regulatory element binding transcription factor 2; sterol regulatory element-binding protein 2; bHLHd2; SREBP2; SREBP-2; class D basic helix-loop-helix protein 2; sterol regulatory element-binding transcription factor 2;
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