Bacterially produced fusion protein encoding 6 consecutive histidines followed by amino acids 32 to 250 of hamster SREBP
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0-Ag14
Clone
IgG-8E5
Cell Line Description
Animals were immunized with a bacterially produced fusion protein encoding 6 consecutive histidines followed by amino acids 32 to 250 of hamster SREBP. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody recognizes both the precursor and mature forms of hamster SREBP-2.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Target
Synonyms
SREBF2; sterol regulatory element binding factor 2; sterol regulatory element-binding protein 2; sterol regulatory element binding protein-2; Sterol regulatory element-binding transcription factor 2; Srebp2; SREBP-2;
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