Mouse Anti-Hamster SCAP Hybridoma [IgG-0E6]

Immunogen

Hamster SCAP (SREBP [sterol regulatory element binding protein] cleavage-activating protein.)

Fusion Species

Mouse X Mouse Hybridoma

Immunological Donor

Mouse spleen

Myeloma

Sp2/0

Clone

IgG-0E6

Cell Line Description

The hybridoma cell line IgG-9D5 secretes a mouse monoclonal antibody (IgG2b) that recognizes hamster SCAP (SREBP [sterol regulatory element binding protein] cleavage-activating protein.) SCAP regulates cholesterol metabolism by stimulating cleavage of the transcription factors SREBP-1 and SREBP-2 in cultured mammalian cells. The antibody is useful in immunoblot and immunoprecipitation of SCAP in cell extracts from cells grown in different conditions; the results help to understand the domain interactions between SREBP and SCAP. The line was produced by fusing Sp2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized against a fusion protein encoding 6 consecutive histidines followed by amino acids 540-707 of hamster SCAP.

Application Notes

WB and IP of SCAP in cell extracts from cells grown in different conditions

Morphology

Lymphoblast

Growth Properties

Suspension

Subculturing

Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml

Storage

Liquid nitrogen vapor phase.

Synonyms

SCAP; SREBF chaperone; sterol regulatory element-binding protein cleavage-activating protein; SREBP cleavage activating protein; SREBP cleavage-activating protein;

Entrez Gene ID