Bacterially expressed full length GST-PTB recombinant protein
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0
Clone
mAb CC8
Cell Line Description
Animals were immunized with bacterially expressed full length GST-PTB recombinant protein. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody is specific for polypyrimidine tract binding protein (PTB). Polypyrimidine tract binding protein (PTB) is a represssor of pre-mRNA splicing.
Application Notes
Immunodepletion experiments.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
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