Mouse Anti-NK Hybridoma [8D7.6.5]

Name: Mouse Anti-NK Hybridoma [8D7.6.5]
SKU: CSC-H0743
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

GeneralInformation

Fusion Species

Mouse X Mouse Hybridoma

Immunological Donor

Mouse spleen

Myeloma

P3X63Ag8.653

Clone

8D7.6.5

Cell Line Description

Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with NC-37 cells but does not inhibit NK cell mediated lysis or lysis by catfish nonspecific cytotoxic cells (NCC) which are analogous to mammalian NK cells.

Morphology

Lymphoblast

Growth Properties

Suspension

CultureMethod

Subculturing

Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml

Storage

Liquid nitrogen vapor phase.

Freezing medium: to complete growth medium, add 10%(v/v) DMSO

Citations

Have you cited CSC-H0743 in a publication? Let us know and earn a reward for your research.

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Effect of pectoral nerve block type II under general anesthesia on the immune function of patients with breast cancer

AMERICAN JOURNAL OF SURGERY

Authors: Cui, Xiuling; Zhu, Cuinv; Chen, Peng; Qu, Min; Zhang, Bowei; Li, Hongtao

Abstract

Background: To investigate whether the use of Pecs II block benefit patients with the respect to the immune functions. Methods: Totally 196 patients were included in this study. These patients were randomized to two groups, general anesthesia alone group (G group) and Pectoral nerve (Pecs) II block under general anesthesia group (PG group). Results: It was found that remifentanil consumption was less in PG group than it in G group. PG group showed a higher proportion of NK cells in peripheral blood mononuclear cell (PBMC) and an improved killing activity than G groups after surgery. We also found that postoperative interleukin (IL)-2 concentration in the plasma of PG group was dramatically higher than it of G group. Interestingly, there was even no significant change between preoperative and postoperative IL-2 levels in PG group, suggesting the less inhibitory effect of Pecs II block on immune system of those patients. Conclusions: In conclusion, these results indicate that Pecs II block use in patients may have an enhanced immunity compared with general anesthesia method. (C) 2020 Elsevier Inc. All rights reserved.

Clonal tracking of haematopoietic cells: insights and clinical implications

BRITISH JOURNAL OF HAEMATOLOGY

Authors: Cordes, Stefan; Wu, Chuanfeng; Dunbar, Cynthia E.

Abstract

Recent advances in high-throughput genomics have enabled the direct tracking of outputs from many cell types, greatly accelerating the study of developmental processes and tissue regeneration. The capacity for long-term self-renewal with multilineage differentiation potential characterises the cellular dynamics of a special set of developmental states that are critical for maintaining homeostasis. In haematopoiesis, the archetypal model for development, lineage-tracing experiments have elucidated the roles of haematopoietic stem cells to ongoing blood production and the importance of long-lived immune cells to immunological memory. An understanding of the biology and clonal dynamics of these cellular fates and states can provide clues to the response of haematopoiesis to ageing, the process of malignant transformation, and are key to designing more efficacious and durable clinical gene and cellular therapies.