Anti-Natural Killer Cell monoclonal antibody, clone CPM56

A polysaccharide (ESPS) purified from Eupolyphaga sinensis Walker by ion exchange chromatography and gel chromatography was investigated, including its structure characterization and antitumor activity. The results showed that ESPS was composed of rhamnose, fucose, arabinose, xylose, glucose, and galactose in a molar ratio of 7.4: 3.1: 13.9: 9.3: 39.7: 26.5, with the mean weight (Mw) of 2.14 x 10(4)Da; the main chain of ESPS was mainly composed of -> 4) alpha D Glcp (1 -> and -> 3) -beta D Galp (1 ->, and the side chains were connected to the main chain through the O-6 atom of glucose and O-4 and O-6 atom of galactose. In addition, ESPS promoted the lymphocyte proliferation and inhibited liver cancer cells growth through enhancing lymphocyte activity in vitro, mainly NK cells. Moreover, ESPS markedly stimulated immunity in H22-bearing mice by increasing the spleen and thymus indices and effectively inhibited H22 cell growth in vivo. These data indicated that ESPS was a polysaccharide component possessing high anti-hepatocellular carcinoma activity, representing a potential immunotherapy candidate for the treatment of liver cancer. (c) 2020 Published by Elsevier B.V.

Acute exacerbations of chronic obstructive pulmonary disease (AECOPD) is a major public health issue. In the study, mass cytometry is used with extensive antibody panels to perform in-depth immune profiling of samples from patients with AECOPD, acute lung infection(AI) and healthy controls. Immune composition of 11 populations including CD4/CD8 T cells, B cells, NK cells, and others is identified. Significantly, 7 T cell subsets are changed among the three groups. Then RNA sequencing at single cell level is further processed to provide a detailed peripheral T cell immunity of AECOPD. Combined analysis suggests that dysfunctional effector T cells in AECOPD are characterized with suppressed mitochondria, dampened mTOR activity, and IFN-gamma production. Furthermore, targeting mTOR pathway synergized the PD-1 blockade therapy promotes T cell glycolysis and IFN-gamma production. The study presents an in-depth immunometabolic atlas of AECOPD.