Animals were immunized with human melanoma cell lines. Spleen cells were fused with P3X63Ag8 myeloma cells. The antibody binds to the 75000 dalton NGF receptor of primates.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 1x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 10%(v/v) DMSO
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