Animals were immunized with fresh prostate cancer (tumor P251). Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with malignant prostatic epithelium but not with benign prostate epithelium.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 2x10^5-1x10^6 cells/ml
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