Live LNCaP cells and purified plasma membranes from LNCaP cells
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
P3X63Ag8.653
Clone
8F22D6
Cell Line Description
Animals were immunized with live LNCaP cells and purified plasma membranes from LNCaP cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts selectively with cells from both normal and neoplastic prostatic epithelium. By immunofluorescence staining, the reaction was much stronger with neoplastic than normal epithelium.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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