Membrane preparations of NIH3T3 cells engineered to overproduce erbB protein
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
P3X8g8.653
Clone
Bc 34.2
Cell Line Description
Animals were immunized with membrane preparations of NIH3T3 cells engineered to overproduce erbB protein. Spleen cells were fused with P3X8g8.653 myeloma cells. The antibody reacts with the extracellular domain of gp185erbB-2. In vitro and in vivo the antibody has no effect upon N87 cells (an erbB overexpressing human gastric carcinoma). The antibody induce autophosphoryaltion of the gp185erbB-2 protein.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
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