NIH3T3 cells transfected with the human c-erb B2 oncogene
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
P3X63Ag8.653
Cell Line Description
Animals were immunized with NIH3T3 cells transfected with the human c-erb B2 oncogene. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The hybridoma cell line named 20.3 produces monoclonal antibody TAb 250. The TAb 250 monoclonal antibody is specific for an extracellular epitope of the c-erbB-2 protein (gp185). It inhibits in a dose-dependent manner the in vitro proliferation of human breast tumor cell lines that overexpress c-erbB-2. Progeny were cured by a 21-day treatment with BM Cycline. The cured cell line is available as CRL-2655. The original patent deposit is available as HB-10646.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
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