Human V-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (avian), ERBB2 ELISA Kit

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

The human ERBB2 ELISA kit is for the quantitative determination of human ERBB2.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.

Contents of Kit

1. Bring all reagents to room temperature before use.
2. Capture Antibody: 0.5 mg/mL of mouse anti-ERBB2 monoclonal antibody. Dilute to a working concentration of 0.5 μg/mL in CBS before coating.
3. Detection Antibody: 0.28 mg/mL of biotinylated rabbit anti-ERBB2 polyclonal antibody. Dilute to a working concentration of 1.0 μg/mL in detection antibody dilution buffer before use.
4. Standard: Each vial contains 14 ng of recombinant ERBB2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°Cto -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1 ng/mL is recommended.
5. Streptavidin-HRP: 50μL of streptavidin conjugated to horse-radish-peroxidase. 1:2,000 Dilution in detection antibody dilution buffer before use.

Storage

Keep streptavidin-HRP at 4°C and protect it from prolonged exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.

Sensitivity

The minimum detectable dose of human ERBB2 was determined to be approximately 15.6 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.