Purified nicotinic acetylcholine receptor of Torpedo californica
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
NS-1
Clone
99C
Cell Line Description
Animals were immunized with purified nicotinic acetylcholine receptor of Torpedo californica. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with determinants on both the gamma and delta subunits of the receptor. And exhibits broad species reactivity (it reacts with receptors of mouse, rat, chicken and frog), and can be used in ultrastructural and immunofluorescence localization and in immunoprecipitation and immunoblotting procedures. The domains recognized by 88B appear to be on the cytoplasmic side of the membrane.
Application Notes
In ultrastructural and IF localization and in IP and WB procedures.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 10%(v/v) DMSO
Citations
Publication ()
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