Mouse Anti-TPO IgG ELISA Kit

Sample

Serum, plasma

Species Reactivity

Mouse

Detection Method

iELISA

Intended Use

The Mouse Anti-TPO IgG ELISA Kit is designed for the Semi-quantitative analysis of Mouse Serum, plasma samples.

Contents of Kit

1. Polystyrene microwell ELISA plates coated with a purified antigen(12-1 x 8 wells), with holder in foil package containing desiccants
2. Negative Control, 1 vial of buffer 1.2 mL
3. Positive Control, 1 vial of buffer 1.2 mL
4. Calibrator A, 1 vial of buffer containing preservative, prediluted, 1.2 mL
5. Calibrator B, 1 vial of buffer containing preservative, prediluted, 1.2 mL
6. Calibrator C, 1 vial of buffer containing preservative, prediluted, 1.2 mL
7. Calibrator D, 1 vial of buffer containing preservative, prediluted, 1.2 mL
8. Sample Diluent, 1 vial – colored straw containing PBS-buffered saline, protein stabilizers and preservative, 50 mL.
9. Antibody Enzyme Conjugate, colored blue containing buffer, protein stabilizers and preservative, 12 mL
10. Wash Buffer (20 ×), 50 mL
11. TMB Chromogen, containing stabilizers, 10 mL
12. Stop Solution, Colorless, 10 mL

Storage

2–8 °C, protected from light and moisture.

Detection Range

0 - 200 U

General Description

Hashimoto's thyroiditis is a chronic autoimmune thyroid disorder characterized by the production of autoantibodies that target thyroid antigens, leading to inflammation and gradual destruction of thyroid tissue. This results in hypothyroidism, with symptoms such as fatigue, weight gain, cold intolerance, constipation, and dry skin. The diagnosis of Hashimoto's thyroiditis relies on clinical presentation and the detection of specific autoantibodies. Key autoantibodies include:
Thyroid Peroxidase Antibodies (TPOAb)
Thyroglobulin Antibodies (TgAb)
Thyroid peroxidase antibodies are also called anti-TPO antibodies or TPO antibodies. Thyroid peroxidase (TPO) antibodies are a type of thyroid antibody which is the key enzyme that catalyzes the iodination and coupling of tyrosine residues during thyroid hormone synthesis. TPOAb can Inhibit TPO enzymatic activity, thereby impairing the synthesis of T4 and T3. Correlate strongly with lymphocytic infiltration of the thyroid gland. Serve as the most sensitive serologic marker of thyroid autoimmunity.