Anti-TPO ELISA Kit

Sample

Serum or plasma.

Species Reactivity

Human

Detection Method

ELISA

Intended Use

The Thyroid Peroxidase (TPO) IgG ELISA Kit is intended for the detection of IgG antibody to Thyroid Peroxidase in human serum or plasma.

Contents of Kit

1. Microwells coated with TPO recombinant Ag 12 x 8 x 1
2. Sample Diluent: 1 bottle (ready to use) 22 ml
3. Calibrator: 1 Vial (ready to use) 1 ml
4. Positive Control: 1 vial (ready to use) 1 ml
5. Negative Control: 1 vial (ready to use) 1 ml
6. Enzyme conjugate: 1 bottle (ready to use) 12 ml
7. TMB Substrate: 1 bottle (ready to use) 12 ml
8. Stop Solution: 1 bottle (ready to use) 12 ml
9. Wash concentrate 20×: 1 bottle 25 ml

Storage

1. Store the kit at 2-8°C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun or strong light during storage or usage.

General Description

Thyroid peroxidase (TPO), is the major autoantigen (933 amino acid residue) in the thyroid microsomal antigen (TMA) particle. The purification and preparation of this antigen has made testing for TMA antibodies obsolete. Assays for TPO antibodies include ELISA, precipitation of radiolabeled TPO-bound autoantibodies with protein A, competition for TPO binding to immobilized anti-TPO murine monoclonal antibodies, autoantibody capture by TPO-coated beads and chemiluminescence. All tests correlate well with detection of TMA. ELISA using TPO recombinant antigen is the most popular assay. Detection of TPO antibodies is strong evidence against a goiter or non-autoimmune causes of hypothyroidism. The annual risk for the development of hypothyroidism is 3% to 4% per year if TPO antibodies are present and TSH is elevated. TPO antibodies are present in 8-9% normal controls, 57-74% patients with Graves disease, 99-100% of Hashimoto disease or idiopathic myxedema, 19% with differentiated thyroid cancer, no patients with subacute thyroiditis and 11% of those with other miscellaneous non-autoimmune thyroid diseases. The prevalence of positive TPO antibodies is higher in elderly (mean age 80 years) women (10%) compared to elderly men (2%). Autoantibody concentration in centenarians also decreases. Studies of TPO epitopes in each domain, A and B, and detection of their specific autoantibodies suggest that the epitope-specific TPO antibodies ratio (A/B) does not change over time in individual patients and that TPO epitope autoantibody patterns may be inherited.

General Notes

The Thyroid Peroxidase (TPO) IgG ELISA Kit is intended for the detection of IgG antibody to Thyroid Peroxidase in human serum or plasma.