Mouse Anti-Rotavirus (EDIM) VP6 IgG ELISA Kit

Sample

Serum or plasma

Species Reactivity

Mouse

Detection Method

iELISA

Intended Use

The Mouse Anti-Rotavirus (EDIM) VP6 IgG ELISA Kit is an immunoassay suitable for detecting and quantifying IgG antibody activity specific for Rotavirus A (Epizootic Diarrhea of Infant Mice: EDIM) VP6 capsid protein in serum or plasma. Other biological fluids, including tissue culture medium, may be validated for use.
This immunoassay is suitable for:
1. Determining immune status relative to non-immune controls
2. Assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization, and timing
3. Qualifying and/or standardizing vaccine batches and protocols
For research use only (RUO), not for diagnosis, cure or prevention of the disease.

Contents of Kit

1. Wash Solution Concentrate (100x), 10ml
2. Sample Diluent Concentrate (20x), 10ml
3. Anti-Mouse IgG HRP Conjugate Concentrate (100x), 0.15ml
4. EDIM Ag Microwell Strip Plate, 8-well strips (12), Coated with recombinant EDIM VP6 antigen, and postcoated with stabilizers
5. Anti-EDIM Sensitivity Control, 0.65 ml, Low level Anti-EDIM, in buffer with detergents and antimicrobial.
6. Anti-EDIM Positive Control, 0.65 ml, High level Anti-EDIM, in buffer with detergents and antimicrobial.
7. Low NSB Sample Diluent, 30 ml, Buffer with protein, detergents and antimicrobial. Use as is for sample dilution.
8. TMB Substrate, 12 ml, Chromogenic substrate for HRP containing TMB and peroxide.
9. Stop Solution, 12 ml, Dilute sulfuric acid.

Storage

The microtiter well plate and all other reagents, if unopened, are stable at 2-8°C until the expiration date printed on the box label.

Sensitivity

The EDIM antigen coating level, HRP conjugate concentration, and Low NSB Sample Diluent are optimized to differentiate antiEDIM IgG from background (non-antibody) signal with mouse serum samples diluted 1:100.

General Description

Rotavirus A (EDIM), a nonenveloped RNA virus, causes severe gastroenteritis in humans and other mammals, including mice and rats, and is readily transmitted via viral shedding in feces. Infection among infants is highly lethal; infected laboratory animals are not suitable for animal research due to compromised immunity, and modified intestinal absorption. Human vaccines using live rotaviruses have been variable in effectiveness. Potentially safer and more efficacious vaccines not composed of live viruses include using recombinant VP6 protein, a highly immunogenic component of the intermediate capsid layer, administered either intranasally or orally along with an adjuvant (e.g., E.coli heat-labile toxin).