A monolayer of MA104 cells is infected with Rotavirus particles. As the infection progresses, rotavirus is released into the culture supernatant. Infectious culture supernatant is collected and Rotavirus is purified from infected cells and the culture med
Store this preparation at -70 °C to -I 00 °C. Repeated freezing and thawing should be avoided. Once thawed, use within 24 hours.
Rotavirus is the most common cause of severe diarrhea among infants and young children. It is a genus of double-stranded RNA virus in the family Reoviridae. Nearly every child in the world has been infected with rotavirus at least once by the age of five. Immunity develops with each infection, so subsequent infections are less severe; adults are rarely affected. There are five species of this virus, referred to as A, B, C, D, and E. Rotavirus A, the most common species, causes more than 90% of rotavirus infections in humans.
This product contains inactivated rotavirus in Bicine/EDTA buffer.
Rotavirus antigen is inactivated using gamma irradiation. This procedure is effective primarily by damaging viral genetic material. Rotavirus inactivation is assessed using a double- passage assay in tissue culture. Rotavirus antigen is inoculated onto an