Sample
Serum, plasma or other biological fluids.
Intended Use
Mouse Anti-Norovirus IgG (MNV1) ELISA Kit is an immunoassay suitable for detecting and quantifying IgG antibody activity specific for Norovirus strain 1 major capsid protein (VP1) antigen in serum or plasma. Other biological fluids, including tissue culture medium, may be validated for use. The kit has no inactivated virus or viral proteins to avoid infection. The antigen used is highly purified recombinant VP1 protein. This kit is for research use only (RUO).
Storage
The microtiter well plate and all other reagents, if unopened, are stable at 2-8°C until the expiration date printed on the box label. Stabilities of the working solutions are indicated under Reagent Preparation.
Precision
Samples and Controls were assayed in duplicate in 5 - 6 separate runs, to provide a measure of between-assay reproducibility. The data are represented using the value of the Sensitivity Control in each assay to calculate a Threshold Index for each control and samples.
Results:
The coefficient of variation (%CV) shows the reproducibility of the assay for measuring one antibody activity (sample or control)
relative to another antibody activity (Sensitivity Control). Variation increases in the threshold region; for this reason, consider running additional tests for borderline samples.
Sensitivity
The MNV antigen coating level, HRP conjugate concentration, and Low NSB Sample Diluent are optimized to differentiate anti-MNV IgG from background (non-antibody) signal with mouse serum samples diluted 1:100. The Anti-Mouse IgG conjugate is blended to equally quantify IgG1 and IgG2a subclass antibody, important when considering difference in subclass emphasis between natural infection and MAP immunization.
General Description
Mouse norovirus (MNV), a non-enveloped ss-RNA virus (Calciviridae) and the most prevalent viral infection in laboratory animal facilities, is highly contagious in causing a mild, persistent enteric infection. MNV replicates in macrophages and dendritic cells, with the potential to alter research data. The major capsid protein, VP1, is highly immunogenic and is implicated in viral virulence in mammals.
MNV infection may be diagnosed by ELISA, measuring rapidly rising antibody titers (8-12 days after infection) to MNV antigen. Mice infected with MNV are not suitable for animal research; in addition to lung changes, MNV may predispose to secondary bacterial infection, cause infertility, and death in susceptible strains. Besides infecting animals, MNV may also contaminate cell lines, transplantable tumors and other biological products; these should be tested by mouse antibody production (MAP), using ELISA to detect anti-MNV after immunization.
Citations
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