Anti-Dec monoclonal antibody, clone 5012

Name: Anti-Dec monoclonal antibody, clone 5012
SKU: DCABH-5539

Mouse anti-Virus Dec monoclonal antibody for ICC/IF, sELISA

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COA

SDS

Datasheet

Summary

Specifications

Antibody Isotype

IgG

Clone

5012

Species Reactivity

Virus

Immunogen

Tissue, cells or virus corresponding to Norovirus. Purified Native Virus.

Conjugate

Unconjugated

Target

Alternative Names

NOV; nephroblastoma overexpressed gene; protein NOV homolog; CCN family member 3; xnov; nov-A;

Entrez Gene ID

373662

UniProt ID

P51609

Product Background

Antigen Description

Norovirus is an RNA virus of the Caliciviridae taxonomic family, causes approximately 90% of epidemic non bacterial outbreaks of gastroenteritis around the world and is responsible for 50% of all foodborne outbreaks of gastroenteritis in the US. Norovirus affects people of all ages. The viruses are transmitted by faecally contaminated food or water and by person to person contact. Noroviruses can genetically be classified into 5 different genogroups (GI, GII, GIII, GIV, and GV) which can be further divided into different genetic clusters or genotypes. For example genogroup II, the most prevalent human genogroup, presently contains 19 genotypes. Genogroups I, II and IV infect humans, whereas genogroup III infects bovine species and genogroup V has recently been isolated in mice. Noroviruses contain a positive-sense RNA genome of approximately 7.5 kbp, encoding a major structural protein (VP1) of about 58~60 kDa and a minor capsid protein (VP2). The virus particles demonstrate an amorphous surface structure when visualized using electron microscopy and are between 27-38 nm in size.

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Documents

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References

Biostimulation of sulfate-reducing bacteria and metallic ions removal from coal mine-impacted water (MIW) using shrimp shell as treatment agent

JOURNAL OF HAZARDOUS MATERIALS

Authors: Rodrigues, Caroline; Nunez-Gomez, Damaris; Dal Magro Follmann, Hiona, V; Silveira, Daniele D.; Nagel-Hassemer, Maria Eliza; Lapolli, Flavio R.; Lobo-Recio, Maria Angeles

Abstract

This paper comprises several assays aiming to identify the basis for the bioremediation of mine-impacted water (MIW). To do so, the conditions for build anoxic microcosms for treating this effluent were varied, containing MIW, and a source of chitin, to biostimulate sulfate-reducing bacteria (SRB). The chitin sources were: commercial chitin (CHIT) and shrimp shell (SS), which in addition to chitin, contains CaCO3, and proteins in its composition. The CHIT assays were not successful in sulfate-reduction, even when the pH was increased with CaCO3. However, in all SS assays the SRB development was successful (85% sulfate removal for assay 3), including the metal-free (MF-SS) assay (75% for assay 5). High-throughput sequencing analysis revealed the structure of bacterial community in the SS assay: the most abundant genera were Clostridium and Klebsiella, both fermentative and chitinase producers; a few SRB from the genera Desulfovibrio and Desulfosporosinus were also detected. In the MF-SS assay, Desulfovibrio genuswas detected but Comamonas was dominant. It could be deduced that SS is a suitable substrate for SRB development, but CHIT is not. The sulfate-reduction process was provided by the cooperation between fermentative/chitinase-producer bacteria together with SRB, which leads to efficient MIW treatment, removing sulfate and metallic ions.

Diazotrophic bacteria and substrates in the growth and nitrogen accumulation of sugarcane seedlings

SCIENTIA AGRICOLA

Authors: Matoso, Ester Schiavon; Reis, Veronica Massena; Giacomini, Sandro Jose; da Silva, Mariana Teixeira; Avancini, Anita Ribas; dos Anjos e Silva, Sergio Delmar

Abstract

Little is known about the interaction between the inoculation of diazotrophic bacteria, the variety, and the substrate used in inoculated sugarcane seedling production. Bearing this in mind, the aim of this study was to evaluate the effects of diazotrophic bacteria inoculation, four sugarcane varieties and four different substrates on the growth and nitrogen accumulation of sugarcane seedlings. Parameters related to sprouting, height, and root growth, as well as nitrogen accumulation, were evaluated. The results indicate that inoculating sugarcane seedlings belonging to the following varieties: RB867515, RB92579, RB966928 and RB975932 with bacteria may increase sprouting speed index, height, root length, fresh and dry matter weight, as well as nitrogen accumulation, which has resulted in a more uniform stem diameter. However, the responses to inoculation differ from variety to variety and are also dependent on the substrate used with better results observed in the commercial substrate and the substrate mixtures containing a higher proportion of organic compost. Understanding the interaction between the variety and the substrate with the bacterial inoculation is essential to the success of the production of inoculated sugarcane seedlings.