Morphine ELISA Kit

Sample

plasma, serum, and urine.

Detection Method

cELISA

Intended Use

Morphine ELISA Kit is a competitive assay that can be used for quantification of morphine in human plasma, serum, and urine. The assay has a range of 16-10,000 pg/ml with a midpoint of approximately 395 pg/ml (50% B/B₀) and a sensitivity(80% B/B₀) of approximately 86 pg/ml.

Precision

Intra-assay precision was determined by analyzing 24 replicates of three matrix controls (urine) in a single assay.

Inter-assay precision was determined by analyzing four replicates of three matrix controls (urine) in 10 separate assays on different days.

Detection Range

16-10,000 pg/ml

Detection Limit

Lower Limit of Detection (LLOD)=3.5 pg/ml

Sensitivity

Sensitivity (defined as 80% B/B₀) = 86.4 pg/ml
Mid-point (defined as 50% B/B₀)= 394.6 pg/ml

General Description

Morphine is an opioid receptor agonist that has been used in the management of severe pain not relieved by non-narcotic analgesics. It is also formed in vivo by the metabolism of codeine and heroin. Common adverse effects of morphine include tolerance development, addiction, and respiratory depression, and it has been associated with misuse and abuse. It has also frequently been implicated in cases of overdose. Morphine is glucuronidated by the UDP-glucuronosyltransferase (UGT) isoforms UGT2B7, UGT1A1, and UGT1A8, and also metabolized by sulfation and N-demethylation, to form the active metabolites morphine-6-glucuronide and normorphine, the toxic metabolite morphine-3-glucuronide, and the inactive metabolites 7,8-dihydromorphine and morphine-3-sulfate. It has an elimination half-life of approximately 1.9 hours following intravenous administration and is excreted primarily in the urine in the form of its metabolites.

Standard Curve

The standard curve presented here is an example of the data typically produced with this kit; however, your results will not be identical to these. You must run a new standard curve.


The sensitivity and mid-point were derived from the standard curve shown above. The standard was diluted in Immunoassay Buffer C (1×).