LPS (Lipopolysaccharides) ELISA Kit

Sample

Serum, plasma, cell culture supernatant and other biological samples

Detection Method

cELISA

Intended Use

In vitro quantitative determination of LPS concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

The sealed kit can be stored at 2-8 °C. The storage condition for opened kit is specified in the table below:
1. ELISA Microplate(Dismountable), 8×12. Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C.
2. Lyophilized Standard, 2vial. Put the lyophilized Standard and Biotin-labeled Antigen (lyophilized) into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C.
3. Biotin-labeled Antigen (Lyophilized), 1vial. Put the lyophilized Standard and Biotin-labeled Antigen (lyophilized) into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C.
4. HRP-Streptavidin Conjugate(SABC, 100×), 120μl. 2-8°C (Avoid Direct Light)
5. TMB Substrate, 10ml. 2-8°C (Avoid Direct Light)
6. 10mM PBS, 200μl. 2-8°C.
7. Sample Dilution Buffer, 20ml. 2-8°C.
8. Antigen Dilution Buffer, 10ml. 2-8°C.
9. SABC Dilution Buffer, 10ml. 2-8°C.
10. Stop Solution, 10ml. 2-8°C.
11. Wash Buffer(25×), 30ml. 2-8°C.
12. Plate Sealer, 5 pieces.
13. Product Description, 1 copy.
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution.

Storage

2-8°C (When unopened, please store the kit at 2-8°C. After the biotin-labeled antigen is dissolved, please divide it into several small packages and freeze them at -20 °C. The biotin-labeled antigen removed from -20 °C should be stored at 2-8°C and used up within 2 weeks.)

Performance Characteristics

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

0.313-20μg/ml

Sensitivity

0.188μg/ml

General Description

Lipopolysaccharide (LPS,CAS: 297-473-0) is located in the outer layer of the membrane of the uncapsulated strain and is exposed to the cell surface. They help maintain the integrity of the outer membrane and prevent bile salts and lipophilic antibiotics from damaging the cells. Lipopolysaccharide (LPS) is a strong activator of immune cells, including B cells, monocytes, macrophages, and other LPS-reactive cells. These immune cells require immunogenic stimuli such as LPS in order to produce cytokines. For example, monocytes activated by LPS can secrete interleukin-6 (IL-6), interleukin-10 (IL-10), or TNF-α.

Standard Curve

The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.

General Notes

Lipopolysaccharide (LPS,CAS: 297-473-0) is located in the outer layer of the membrane of the uncapsulated strain and is exposed to the cell surface. They help maintain the integrity of the outer membrane and prevent bile salts and lipophilic antibiotics from damaging the cells. Lipopolysaccharide (LPS) is a strong activator of immune cells, including B cells, monocytes, macrophages, and other LPS-reactive cells. These immune cells require immunogenic stimuli such as LPS in order to produce cytokines. For example, monocytes activated by LPS can secrete interleukin-6 (IL-6), interleukin-10 (IL-10), or TNF-α.