Simultaneous Detection of Salmonella typhimurium and Escherichia coli O157:H7 in Drinking Water and Milk with Mach-Zehnder Interferometers Monolithically Integrated on Silicon Chips
Michailia Angelopoulou, Panagiota Petrou, Konstantinos Misiakos, Ioannis Raptis, Sotirios Kakabakos
Applications: Unspecified application
Reactive species: E. coli O157:H7
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Abstract: The consumption of water and milk contaminated with bacteria can lead to foodborne disease outbreaks. For this reason, the development of rapid and sensitive analytical methods for bacteria detection is of primary importance for public health protection. Here, a miniaturized immunosensor based on broadband Mach-Zehnder Interferometry for the simultaneous determination of S. typhimurium and E. coli O157:H7 in drinking water and milk is presented. For the assay, mixtures of bacteria solutions with anti-bacteria-specific antibodies were run over the chip, followed by solutions of biotinylated anti-species-specific antibody and streptavidin. The assay was fast (10 min for water, 15 min for milk), accurate, sensitive (LOD: 40 cfu/mL for S. typhimurium; 110 cfu/mL for E. coli) and reproducible. The analytical characteristics achieved combined with the small chip size make the proposed biosensor suitable for on-site bacteria determination in drinking water and milk samples."
Article snippet:
Lipopolysaccharide (LPS) of E. coli was obtained from Creative Diagnostics (Shirley, NY, USA).
Figure 1. Percent signal values obtained for a calibrator containing 25 (yellow columns) or 250 ng/mL of E. coli LPS (orange columns) with respect to zero calibrator (red columns) without pre-incubation and with 5-, 10-, 15-, 30- and 60-min pre-incubation of the calibrators with anti-E. coli antibody solution
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