It is recommended that this material be shored at 2-8°C prior to and following reconstitution.
Reconstitution
LPS is dispersable in aqueous solvents at concentrations of 1mg/ml. To achieve suspension in water, heating to about 50°C with intermittent vortexing or sonication is generally recommended. Allow ample time for dispersion to occur. The use of 0.5% triethylamine aids in dispersion. Triethylaine is very basic and may be neutralized with Tris HCl to avoid hydrolysis of the LPS fatty acid chains.
Antigen Description
This is a highly potent lipopolysaccharide produced from E. coli O113. This endotoxin has been purified to eliminate residual intrinsic proteins and nucleic acids. Potency of this product, in endotoxin units (EU), is determined to be at least 10 EU per ng LPS, or 106 EU per mg LPS.
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