Tag/Conjugate
Unconjugated
Endotoxin
Endotoxin activity by a kinetic chromogenic LAL assay: 2.7 EU/ng
Alternative Names
Escherichia coli Lipopolysaccharides
Format
Lyophilized in water
Storage
It is recommended that this material be shored at 2-8°C prior to and following reconstitution.
Reconstitution
LPS is dispersable in aqueous solvents at concentrations of 1mg/ml. To achieve suspension in water, heating to about 50°C with intermittent vortexing or sonication is generally recommended. Allow ample time for dispersion to occur. The use of 0.5% triethylamine aids in dispersion. Triethylaine is very basic and may be neutralized with Tris HCl to avoid hydrolysis of the LPS fatty acid chains.
Citations
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RICE, GC; ROSEN, J; et al. CT-1501R SELECTIVELY INHIBITS INDUCED INFLAMMATORY MONOKINES IN HUMAN WHOLE-BLOOD EX-VIVO. SHOCK 1:254-266(1994).
Ma, SY; Zhang, YP; et al. Preparation of Immunoglobulin Y (IgY) Against Lipopolysaccharide using Gel Chromatography from the Yolks of Eggs Laid by Immunized Hens. PROTEIN JOURNAL 29:475-480(2010).
COA
Certificate of Analysis
