E. coli J5 LPS, native protein from E. coli J5
Endotoxin activity by a kinetic chromogenic LAL assay: 2.7 EU/ng
Escherichia coli Lipopolysaccharides
Lyophilized in water
It is recommended that this material be shored at 2-8°C prior to and following reconstitution.
LPS is dispersable in aqueous solvents at concentrations of 1mg/ml. To achieve suspension in water, heating to about 50°C with intermittent vortexing or sonication is generally recommended. Allow ample time for dispersion to occur. The use of 0.5% triethylamine aids in dispersion. Triethylaine is very basic and may be neutralized with Tris HCl to avoid hydrolysis of the LPS fatty acid chains.