Human MPO(Myeloperoxidase) ELISA Kit

Sample

Serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Human

Detection Method

sELISA

Intended Use

In vitro quantitative determination of MPO concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

Storage

Store at 2-8°C (for sealed box), please do not freeze! See kit label for expiry date.
Stability
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

0.156-10 ng/mL

Sensitivity

0.062ng/mL

General Description

Myeloperoxidase (MPO) is an enzyme found in myeloid white blood cells. MPO is encoded by the MPO gene and highly expressed in myeloid cells. It plays a role in immune responses such as inflammation and infection by catalyzing oxidation reactions to produce strong oxidants such as hypochlorous acid, hydrogen peroxide, and hydroxyl radicals, which can kill and degrade invading microorganisms.MPO is also associated with certain diseases such as atherosclerosis, myocardial infarction, inflammatory bowel disease, and neurodegenerative diseases. Excessive expression of MPO and the oxidants generated by MPO catalysis may cause cellular damage and inflammatory reactions, thereby accelerating disease progression.

Standard Curve

This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C - 25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.)
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.

General Notes

Myeloperoxidase (MPO) is an enzyme found in myeloid white blood cells. MPO is encoded by the MPO gene and highly expressed in myeloid cells. It plays a role in immune responses such as inflammation and infection by catalyzing oxidation reactions to produce strong oxidants such as hypochlorous acid, hydrogen peroxide, and hydroxyl radicals, which can kill and degrade invading microorganisms.MPO is also associated with certain diseases such as atherosclerosis, myocardial infarction, inflammatory bowel disease, and neurodegenerative diseases. Excessive expression of MPO and the oxidants generated by MPO catalysis may cause cellular damage and inflammatory reactions, thereby accelerating disease progression. In vitro quantitative determination of MPO concentrations in serum, plasma, cell culture supernatant and other biological samples.