Recombinant Human GPC3 (a.a. 25-559 ) Protein [His]

Product Overview

Human Glypican 3, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).

Nature

Recombinant

Predicted N terminal

Gln 25 & Ser 359

Tag/Conjugate

His

Molecular Weight

This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 24.6 kDa respectively. The protein migrates as 30 kDa, 40 kDa and 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
SDS-PAGE: 34 kDa, reducing conditions

Bio-activity

Immobilized Human GPC3 (cat. DAGC-Y26001) at 0.5 μg/mL can bind Anti-Human GPC3 MAb with a linear range of 0.1-12.5 ng/mL.
Anti-human GPC3 MAb captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human GPC3 (cat. DAGC-Y26001) with an affinity constant of 1.33 nM as determined in a SPR assay.
Loaded Anti-Human GPC3 MAb on AHC Biosensor, can bind Human GPC3 (cat. DAGC-Y26001) with an affinity constant of 1.48 nM as determined in BLI assay.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Alternative Names

GPC3; OCI5; Glypican-3; GTR2-2; MXR7; DGSX; SDYS; SGB; SGBS; SGBS1

Purity

>90% as determined by SDS-PAGE
>95% as determined by SEC-MALS

Format

Lyophilized

Buffer

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Preservative

None

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Introduction

GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.

Keywords

GPC3; OCI5; Glypican-3; GTR2-2; MXR7; DGSX; SDYS; SGB; SGBS; SGBS1