Human GPC3 ELISA Kit

Sample

serum, urine, cell culture supernates

Species Reactivity

Human

Intended Use

This Kit is for the quantitative determination of human glypican-3 (GPC-3) concentrations in serum, urine, cell culture supernates.

Contents of Kit

1. Assay plate (12 x 8 coated Microwells) 1(96 wells)
2. Standard (Freeze dried) 2
3. Biotin-antibody (100 x concentrate) 1 x 120 μl
4. HRP-avidin (100 x concentrate) 1 x 120 μl
5. Biotin-antibody Diluent 1 x 15 ml
6. HRP-avidin Diluent 1 x 15 ml
7. Sample Diluent 1 x 50 ml
8. Wash Buffer (25 x concentrate) 1 x 20 ml
9. TMB Substrate 1 x 10 ml
10. Stop Solution 1 x 10 ml
11. Adhesive Strip (For 96 wells) 4
12. Instruction manual 1

Storage

Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. Opened kit Coated assay plate May be stored for up to 1 month at 2 - 8°C. Try to keep it in a sealed aluminum foil bag, and avoid the damp. Standard/ Biotin-antibdy/ HRP-avidin May be stored for up to 1 month at 2 - 8°C. If don't make recent use, better keep it store at -20°C. Biotin-antibody Diluent/ HRP avidin Diluent/ Sample Diluent/ Wash Buffer/ TMB Substrate/ Stop Solution be stored for up to 1 month at 2 - 8°C.

Precision

##Intra-assay Precision (Precision within an assay): CV%<8%##
Three samples of known concentration were tested twenty times on one plate to assess.
##Inter-assay Precision (Precision between assays): CV%<10%##
Three samples of known concentration were tested in twenty assays to assess.

Detection Range

0.625 ng/ml-40 ng/ml

Sensitivity

The minimum detectable dose of human GPC-3 is typically less than 0.156 ng/ml.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Standard Curve