Human EFHD2 (EF-hand domain-containing protein D2) ELISA Kit (DEIA-FN437)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma, cell culture supernatants, tissue homogenate
Species Reactivity
Human
Intended Use
For quantitative detection of Human EFHD2 (EF-hand domain-containing protein D2) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
0.312-20 ng/mL
Sensitivity
0.188 ng/mL
Standard Curve

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References


Kroczek, C; Lang, C; et al. Swiprosin-1/EFhd2 Controls B Cell Receptor Signaling through the Assembly of the B Cell Receptor, Syk, and Phospholipase C gamma 2 in Membrane Rafts. JOURNAL OF IMMUNOLOGY 184:3665-3676(2010).
Brachs, S; Turqueti-Neves, A; et al. Swiprosin-1/EFhd2 limits germinal center responses and humoral type 2 immunity. EUROPEAN JOURNAL OF IMMUNOLOGY 44:3206-3219(2014).

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