Human CMV IgG ELISA RUO

Sample

serum and plasma (EDTA, lithium heparin or citrate plasma)

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The CMV IgG ELISA provides materials for the quantitative and qualitative determination of IgG-class antibodies to Cytomegalovirus (CMV) in human serum and plasma (EDTA, lithium heparin or citrate plasma).This assay is intended for research use only.

Contents of Kit

1. Microtiterwells, 12 x 8 (break apart) strips, 96 wells; Wells coated with inactivated grade 2 Cytomegalovirus (CMV) antigen (strain AD-169). (incl. 1 cover foil)
2. Sample Diluent *, 1 vial, 100 mL, ready to use,colored yellow; pH 7.2 ± 0.2.
3. Standard (Standard 1 - 3)*, 3 vials, ready to use, Concentrations: 10, 40, 80 DU/mL,
Standard 1, 2.0 mL, colored green, green cap
Standard 2, 1.0 mL, colored blue, blue cap
Standard 3, 1.0 mL, colored red, red cap
4. Pos. Control *, 1 vial, 1.0 mL, ready to use;colored purple, black cap.
5. Neg. Control *, 1 vial, 2.0 mL, ready to use;colored yellow, yellow cap.
6. Enzyme Conjugate *, 1 vial, 20 mL, ready to use,colored red, antibody to human IgG conjugated to horseradish peroxidase.
7. Substrate Solution, 1 vial, 14 mL, ready to use,Tetramethylbenzidine (TMB).
8. Stop Solution, 1 vial, 14 mL, ready to use,contains 0.2 mol/L H₂SO₄, Avoid contact with the stop solution. It may cause skin irritations and burns.
9. Wash Solution *, 1 vial, 30 mL (20× concentrated for 600 mL), pH 6.5 ± 0.1
* contain non-mercury preservative

Storage

When stored at 2 °C to 8 °C unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
Opened reagents must be stored at 2 °C to 8 °C. Microtiter wells must be stored at 2 °C to 8 °C. Once the foil bag has been opened, care should be taken to close it tightly again.
Opened kits retain activity for 8 weeks if stored as described above.

Detection Range

1.18 – 80 DU/mL