Cytomegalovirus strain AD-169 is grown in MRC-5 cells and harvested under conditions that ensure full viral protein expression. Infected cells are collected in glycine-buffered saline, disrupted to release CMV antigens, clarified by centrifugation, and inactivated by gamma irradiation.
Nature
Native
Format
Liquid
Buffer
Glycine buffered saline, pH 9.5
Preservative
None
Storage
Store at -80°C. Avoid multiple freeze/thaw cycles.
Keywords
Cytomegalovirus; CMV; HCMV; HHV-5
Inactivation
Gamma-irradiated
Citations
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Q: Hello, Would you be able please to tell me in which type of buffer this is diluted and whether this would be suitable for cell culture? Best wishes
A: Buffer used for dilution/suspension: NTE buffer (NaCl, Tris, EDTA) + sucrose. Suitability: Intended for immuno-detection of antigens; may not suitable for cell culture or infection assays.
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