Infected cells are harvested, washed and virus extracted by alkaline treatment and sonication.
Target
CMV
Nature
Native
Tag/Conjugate
Unconjugated
Alternative Names
CMV; Cytomegalovirus
Format
Liquid
Concentration
mg/ml by Bradford (Biorad) assay using BSA as standard
Buffer
100mM glycine buffer, pH 9.5
Preservative
None
Storage
Frozen (-70°C). Sonicate before use. Avoid repeated freezing and thawing
Introduction
Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein–Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period.
Keywords
CMV; Cytomegalovirus
Inactivation
By treatment with β-propriolactone. Infectivity confirmed negative by absence of growth under original cell culture conditions.
Citations
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