Cytomegalovirus IgG ELISA Kit (DEIA326)

Regulatory status: For research use only, not for use in diagnostic procedures.

Write a review

Size
96T
Sample
serum, plasma
Species Reactivity
Human
Intended Use
Enzyme immunoassay for the quantitative determination of IgG antibodies against Cytomegalovirus in human serum and plasma.
Contents of Kit
1. Microtiter Plate: 1 x 12 x 8.
2. Enzyme Conjugate IgG: 1 x 15 mL.
3. Standard A-D: 4 x 2 mL.
4. Diluent Buffer: 1 x 60 mL.
5. Wash Buffer, Concentrate (10x): 1 x 60 mL.
6. TMB Substrate Solution: 1 x 15 mL.
7. TMB Stop Solution: 1 x 15 mL.
8. Adhesive Foil: 2 x.
9. Plastic Bag: 1 x.
Storage
The kit is shipped at ambient temperature and should be stored at 2-8°C. Keep away from heat or direct sun light. The storage and stability of specimen and prepared reagents is stated in the corresponding chapters. The unopened reagents are stable until the expiry date indicated. The Kit is stable up to 3 months after the first opening when the Microtiterplate is packed in a tightly closed bag, the bottles are closed with their screw caps and the kit is stored at 2-8°C.
Performance Characteristics
General Description
Cytomegaly is caused by a viral infection, which shows an inapparent progression, however leads to serious symptoms with immune-compromised patients and newborns.

The cytomegaly virus (CMV) with a particle diameter of 100 nm (inclusive envelope the virion measures 200 nm) belongs to the family of herpesviridae like Herpes 1 and Herpes 2. The transmission is effected via saliva and by long-term intimate contacts. The incubation time is 2-10 days for primary infections.

Cytomegaly appears often during the gravidity, and per year up to 5% of the pregnant women show clinical signs of the disease. In the early phase the illness frequently remains undetected, and the affected women themselves show no symptoms. When however newborns are infected (1% of the births), these show in 10% of the cases disease symptoms with late damages like retinitis, ulcera of the mucous membrane, viral pneumonia, meningitis, microencephaly, hydrocephalus and skin ulcerations. The incidence of women capable of childbearing is estimated to be 65%, so that an assessment of the immune status before pregnancy is mandatory. After a negative result, a control check is recommended in the 20th week of gestation.

Besides the symptomatic treatment a specific therapy with phosphonoformate and ganciclovir is possible. A vaccination does not exist, so that in the case of an infection only passive CMV immunoglobulins are administered.

Together with the differential assessment of the above mentioned symptoms, the diagnosis is performed clinically in tissue samples via the so-called "owl eyes". The following laboratory methods are available: CF and ELISA, determination of virus nucleic acid (PCR) as well as virus isolation (cell culture). Serologically, IgG and IgM antibodies can be detected and quantitated by the ELISA technique. Also the measurement of the avidity of the IgG molecules is appropriate.
Standard Curve

Citations


Have you cited DEIA326 in a publication? Let us know and earn a reward for your research.

Related Products


Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket

Noire, M. D. A. (2015). Séroprévalence de l’infection au cytomégalovirus chez les donneurs de sang à l’hôpital de district de Bonassama Douala-Cameroun. Médecine d’Afrique Noire, 62(7), 374.

Have you cited DEIA326 in a publication? Let us know and earn a reward for your research.

Online Inquiry

Name:
Phone: *
E-mail Address: *
Service & Products Interested: *
Project Description:
Verification Code: * Please input "diagnostics" as verification code.

Online Inquiry

  Interested in larger quantities ? request a quote!
  Protocol may be improved. Please feel free to contact us to obtain the latest version.!

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

OUR PROMISE TO YOU Guaranteed product quality expert customer support

Inquiry Basket