Human Anti-Gal d 2 IgE ELISA Kit

Sample

Serum

Species Reactivity

Human

Detection Method

ELISA

Intended Use

This kit is intended for the quantitative detection of specific IgE antibodies against Gal d 2 in human serum.

Contents of Kit

1. 1×96 Microplate coated with anti-IgE antibody (1.5~3.0 μg/mL anti-IgE antibody coated on the microplate)
2. 1×16 mL HRP-streptavidin conjugate solution (1:4000~1:20000 horseradish peroxidase-streptavidin dissolved in 0.05M Tris-HCl buffer, pH 7.2±0.3)
3. 1×50 mL Concentrated washing buffer (25x) (10% w/v Tween-20 dissolved in 0.03M phosphate buffer, pH 7.2±0.3)
4. 1×16 mL Substrate solution (TMB) (TMB dissolved in buffer, pH 3.55±0.2)
5. 1×16 mL Stop solution (0.5M H₂SO₄)
6. 4×2.8 mL Allergen-biotin (allergen-biotin dissolved in 0.05M Tris-HCl buffer, pH 7.2±0.3)

Storage

Store at 2~8°C, with a validity of 12 months.
The production date and expiration date are indicated on the label.
After opening, the validity is 28 days when stored at 2~8°C.
All reagents should be stored at 2~8°C when not in use to avoid repeated opening of the packaging. Unused microplate strips should be placed back into the aluminum foil bag, sealed, and stored with the desiccant.
Do not use expired kits.

Performance Characteristics

1. Reference samples: The detection results of negative enterprise reference samples please refer to COA; the detection rate of the limit of detection reference samples is more than 90%; the CV of the repeatability reference samples is less than ±15%; after serial dilution of the linearity reference samples, the detection linearity r value is greater than 0.99.
2. Lower limit of detection: please refer to COA.
3. Precision: For negative reference samples and lower limit of detection reference samples, statistics were performed for 20 days within batches, between batches, within days, and between days. The precision of negative reference samples is not higher than 30%, and the precision of the lower limit of detection is not higher than 15%.
4. Linear range: High-value samples were serially diluted to at least 5 concentrations, and the linear correlation coefficient r > 0.9900 was obtained after detection and fitting within the concentration range of 0-100 IU/mL.
5. Repeatability: At least two concentration levels of samples were repeatedly tested 10 times, and the variation coefficient CV ≤ 15%.
6. Batch-to-batch difference: Three batch reagent kits were used to test the same sample, and the batch-to-batch variation coefficient CV ≤ 15%.