Cytomegalovirus IgG ELISA kit

Sample

Serum, plasma or cerebrospinal fluid

Species Reactivity

Human

Detection Method

iELISA

Intended Use

Cytomegalovirus IgG is quantitative and qualitative test for detection of human antibodies in serum, plasma or cerebrospinal fluid against Cytomegalovirus. For sale in the U.S. for Research Use Only. Not for use in diagnostic procedures.

Contents of Kit

1. ELISA Microplate:
Break apart microtiter test strips each with eight antigen coated single wells.
The coating material is inactivated. 12 × 8
2. Standard serum (ready-to-use)
Human serum in protein containing phosphate buffer; negative for anti-HIV Ab, HBsAg (Hepatitis B-Virus surface antigen) and anti-HCV Ab;
preservative: < 0.1 % sodium azide; colouring: Amaranth O. 2 × 2 ml
3. Negative control serum (ready-to-use)
Human serum in protein containing phosphate buffer; negative for anti-HIV Ab, HBsAg (Hepatitis B-Virus surface antigen) and anti-HCV Ab;
preservative: < 0.1 % sodium azide; colouring: Lissamin Green V. 2 ml
4. Anti-human IgG conjugate (ready-to-use)
Anti-human IgG polyclonal antibody, conjugated to alkaline phosphatase, stabilised with protein stabilisation solution;
preservative: < 0.1 % methylisothiazolone, < 0.1 % bromnitrodioxane. 13 ml
5. Washing solution concentrate (sufficient for 1000 ml)
Sodium chloride solution with Tween 20 and 30 mM Tris/HCl, pH 7.4;
preservative: < 0.1 % sodium azide. 33.3 ml
6. Dilution buffer (ready-to-use)
Protein containing phosphate buffer with Tween 20;
preservative: < 0.1 % sodium azide; colouring: 0.01 g/l Bromphenol blue. 2 × 50 ml
7. Stopping solution (ready-to-use)
< 0.1 N sodium hydroxide, 40 mM EDTA. 15 ml
8. Substrate (ready-to-use)
Para-nitrophenylphosphate in solvent free buffer;
preservative: < 0.1 % sodium azide. 13 ml

Storage

2-8°C

General Description

The Cytomegalovirus (CMV), a DNA virus, is a member of the Human Herpesvirus group which is pathogenic for humans.
Primary infection may result from contact with saliva, genital secretions, urine and breast milk of infected persons, as well as transplacentally or iatrogenically (transfusions, transplantations). After primary infection a lifelong latent infection ensues. The virus resides in lymphocytes where it may be reactivated under certain circumstances. The frequencies of infection in developed and underdeveloped countries are between 45% and 50%, and over 90%, respectively