Product Overview
Human CD73 (a.a 27-549) protein was expressed in HEK293 cells and fused to 6xHis at the C-terminus
Molecular Weight
58.8 kDa
Alternative Names
Ecto-5'-nucleotidase; 5'-NT; CD73
Purity
> 95% , as determined by SDS-PAGE
Concentration
Batch dependent - please inquire should you have specific requirements.
Buffer
PBS with 20% glycerol
Storage
Store at -20°C to -80°C. Avoid multiple freeze/thaw cycles.
Introduction
Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.
Keywords
NT5E; CD73; Enzyme
Citations
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