Anti-NT5E Polyclonal Antibody (CABT-L2129) Knockout Validated

Rabbit Anti-Human NT5E (Ser48~val527) Polyclonal Antibody for WB

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human, Bovine
Immunogen
Recombinant fragment corresponding to human NT5E (Ser48~val527)
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution: WB: 0.5-2 ug/ml
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Knockout Varification: Lane 1: Wild-type K562 cell lysate. Lane 2: NT5E knockout K562 cell lysate. Predicted MW: 63kd, Observed MW: 70kd
Western Blot. Sample. Bovine Cerebrum lysate.
Western Blot. Sample. Bovine Liver lysate.
Western Blot. Sample. Human K562 cell lysate.

Target


Alternative Names
CD73; NT5-E; E5NT; E5-NT; NTE; EN
Entrez Gene ID
UniProt ID

Product Background


Pathway
HIF-1-alpha transcription factor network; Metabolic pathways; Metabolism; Metabolism of nucleotides; Nicotinate and nicotinamide metabolism; Purine catabolism; Purine metabolism; Pyrimidine catabolism;

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Lakowski, J; Han, YT; et al. Effective Transplantation of Photoreceptor Precursor Cells Selected Via Cell Surface Antigen Expression. STEM CELLS 29:1391-1404(2011).
Snider, NT; Altshuler, PJ; et al. Alternative splicing of human NT5E in cirrhosis and hepatocellular carcinoma produces a negative regulator of ecto-5 '-nucleotidase (CD73). MOLECULAR BIOLOGY OF THE CELL 25:4024-4033(2014).

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